Buy Acridine orange base (494-38-2) MF: C17H19N3 | MW: 265.35. Get High Quality Acridine orange base (494-38-2) from Tristains at Dawnscientific.com
Acridine Orange Base, a fluorescent dye, has several applications in biological and biomedical research. A member of the class of aminoacridines that is acridine carrying two dimethylamino substituents at positions 3 and 6. The hydrochloride salt is the fluorescent dye ‘acridine orange’, used for cell cycle determination. It is also known as Solvent Orange 15. Its stability at low pH allows it to penetrate even acidic organelles in order to bind to DNA or RNA.
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Applications
- It is used in a wide variety of biotechnology applications, including microscopy and flow cytometry.
- Acridine orange has also been used as a lysosomal dye. It is widely used to detect the presence of homeopathic potencies.
- Acridine Orange Base is used to assess cell viability and cytotoxicity. It can distinguish between live cells (green fluorescence) and apoptotic or dead cells (orange-red fluorescence) based on their staining patterns.
- Acridine Orange Base is commonly used for staining nucleic acids, including DNA and RNA, in cells and tissues. It intercalates into the nucleic acid strands and emits fluorescence, allowing for the visualization and analysis of DNA or RNA distribution, integrity, and cellular localization.DNA/RNA Quantification.
Benefits
- Gives accurate, reliable, and reproducible results
- Quantifying nucleic acids in solution
- Its stable at low pH
- Used in cell cycle assays
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