Buy Acridine orange (10127-02-3) MF: C17H19ClN3•HCl•1/2ZnCl2 | MW: 369.96. Get High Quality Acridine orange (10127-02-3) from Tristains at Dawnscientific.com
Acridine Orange is a cell-permeant nucleic acid binding dye that emits green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA or RNA. When acridine orange binds to DNA, the dye exhibits a maximum excitation at 502 nm producing a maximum emission of 525 nm. When bound to RNA, acridine orange displays a maximum emission value of 650 nm and a maximum excitation value of 460 nm. It is also known to be a positive solvatochromic dye. Acridine orange has also been used as a lysosomal dye. The other names of it are 3,6-Acridinediamine, Acridine Orange Base, Acridine Orange NO, Basic Orange 14 etc. Acridine Orange is commonly used as a fluorescent stain in microbiology and histology.
“TriStains”, Biological Stains that offer range of stains used in Histology, Cytology, Microbiology and Hematology laboratories. TriStains meet the highest quality standards and give excellent color performance of desired components of cells and tissue in life science laboratories. TriStains series products are carefully tested to ensure accurate, reliable, and reproducible results. Our products are available in different packaging sizes to allow you to get all types of stains & Indicators for your specific purposes from a single source.
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- Acridine orange is useful for cell-cycle studies.
- It may also be useful as a method for measuring apoptosis, and for detecting intracellular pH gradients and the measurement of proton-pump activity2. This procedure optimally stains cells for analysis by flow cytometry.
- Acridine orange has been widely accepted and used in many different areas, such as epifluorescence microscopy, and the assessment of sperm chromatin quality.
- Acridine orange staining has to be performed at an acidic pH to obtain the differential staining, which allows bacterial cells to stain orange and tissue components to stain yellow or green.
- Able to withstand low pH environments
- Differentiate various types of cells (i.e., bacterial cells and white blood cells)
- High quality and good effectiveness