Buy Bromophenol Blue Free Acid (115-39-9) MF: C19H10Br4O5S | MW: 669.96. Get High Quality Bromophenol Blue Free Acid (115-39-9) from Tristains at Dawnscientific.com
Bromophenol Blue Free Acid is a synthetic dye belonging to the class of sulfonphthalein dyes. It is an acidic form of bromophenol blue and is often used as a pH indicator.
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Applications :
pH Indicator: Bromophenol Blue Free Acid is primarily utilized as a pH indicator due to its color-changing properties. It transitions from yellow in acidic solutions to blue in alkaline solutions. The transition range typically lies between pH 3.0 and 4.6.Gel Electrophoresis: Gel electrophoresis is a widely used technique in molecular biology for the separation and analysis of nucleic acids and proteins. Bromophenol Blue Free Acid is commonly added to DNA or RNA samples to track the progress of electrophoresis. It migrates through the gel alongside the nucleic acids and provides a visible front that indicates the migration distance.Buffers and Reagents: Bromophenol Blue Free Acid can be included in buffer solutions used in various biochemical and molecular biology assays. It aids in visualizing the buffer during preparation, mixing, and pipetting, ensuring accurate measurements. Additionally, it can be added to certain reagents to assist in the visualization of reaction progress or completion.Educational and Research Applications: Bromophenol Blue Free Acid is widely used in educational laboratories and research settings to teach and study pH concepts, acid-base chemistry, and molecular biology techniques. Its distinct color change and ease of use make it a valuable tool for demonstrating various scientific principles.Stain for Protein Detection: In protein biology, Bromophenol Blue Free Acid can be used as a stain to visualize protein bands on polyacrylamide gels. After electrophoresis, proteins can be stained with bromophenol blue, allowing researchers to detect the presence, relative quantity, and migration pattern of proteins.Microbiology: Bromophenol Blue Free Acid can also be used in microbiology to differentiate bacteria based on their ability to ferment carbohydrates. It can be incorporated into specific growth media to detect acid production resulting from carbohydrate metabolism. The color change of the indicator helps identify different types of bacteria.
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