Buy Bromophenol Blue Sodium Salt (34725-61-6) MF: C19H9Br4O5S•Na | MW: 691.94. Get High Quality Bromophenol Blue Sodium Salt (34725-61-6) from Tristains at Dawnscientific.com
Bromophenol Blue Sodium Salt is a synthetic dye commonly used as a pH indicator and staining agent in various scientific applications. It belongs to the class of sulfonphthalein dyes and is the sodium salt form of bromophenol blue.
Bromophenol Blue Sodium Salt is a blue-colored compound that undergoes a color change depending on the pH of the solution it is present in. It is water-soluble and often used as a more soluble alternative to the free acid form of bromophenol blue.
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Applications :
pH Indicator: Bromophenol Blue Sodium Salt is widely used as a pH indicator due to its pH-sensitive color change. It transitions from yellow in acidic solutions to blue in alkaline solutions. The transition range typically lies between pH 3.0 and 4.6. It is commonly employed in laboratory settings to monitor pH changes in chemical reactions or to determine the acidity or alkalinity of various solutions.Gel Electrophoresis: Bromophenol Blue Sodium Salt is commonly used as a tracking dye in gel electrophoresis. It is added to nucleic acid samples, such as DNA or RNA, to visualize their migration during electrophoresis. The blue color of the dye allows researchers to monitor the progress of the separation and determine the approximate location of the DNA or RNA bands.Protein Staining: Bromophenol Blue Sodium Salt can be used as a protein stain in polyacrylamide gel electrophoresis (PAGE) and protein assays. It binds to proteins, enabling their visualization and detection. By staining protein bands with bromophenol blue, researchers can analyze the presence, quantity, and migration pattern of proteins in biological samples.Biochemical Assays: In various biochemical assays, Bromophenol Blue Sodium Salt can be employed as an indicator to monitor enzymatic reactions or to determine the completion of certain chemical reactions. The color change associated with pH variations helps in assessing the progress and outcome of the assay.Microbiology: Bromophenol Blue Sodium Salt can be incorporated into specific growth media in microbiology to detect pH changes resulting from bacterial metabolic activities. It helps in differentiating bacteria based on their ability to ferment carbohydrates and produce acid. The color change of the indicator aids in the identification of different bacterial species.Education and Research: Bromophenol Blue Sodium Salt is often used in educational laboratories and research settings to teach and study pH concepts, molecular biology techniques, and protein analysis methods. Its distinct color change and ease of use make it a valuable tool for demonstrating scientific principles and conducting experiments.
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