Fast Green F.C.F. Certified CAS: 2353-45-9 | MF: C37H34N2O10S3Na2 | MW: 808.85, Buy Histological Stains & Dyes for Histology, Cytology, Microbiology, Hematology & Biology Lab from TriStains at Dawnscientific.
Fast Green FCF Certified, also known as C.I. 42053 or Food Green 3 (CAS No. 2353-45-9), is a water-soluble, synthetic triarylmethane dye colorant. This crystalline, dark green powder is widely utilized as a food coloring additive. Its chemical formula is C37H34N2O10S3Na2. The dye is certified for the safranin-fast green FCF stain for plant cells and as a substitute for aniline blue in Gomori’s one-step trichrome procedure. It is a sensitive stain for proteins in polyacrylamide gels, particularly suitable for isoelectric focusing. Also, Fast Green FCF is used as a cytological counterstain and a mammalian tissue stain for collagen and muscle.
Applications of Fast Green F.C.F. Certified
- Histology and Cytology Staining: Regular Fast Green FCF Certified is used routinely in histology as a plant cell stain in combination with other stains like Safranin. The safranin-fast green FCF stain has been found useful in observing plant tissue structures and cellular details under the microscope. Also, Fast Green FCF is used in staining collagen deposits and also in the process of staining proteins and starch, thus improving the contrast and visualization of the tissues.
- Gomori’s One Step Trichrome Staining: In histology, Fast Green FCF Certified is used instead of aniline blue for Gomori’s one-step trichrome procedure, which is one of the commonly used techniques for differentiating muscle fibers from collagen and other tissues. Its green color enables effective identification of various tissue elements.
- Protein Staining in Electrophoresis: Fast Green FCF Certified is a particularly useful stain for proteins in isoelectric focusing (IEF) as well as in native PAGE and SDS-PAGE.
- Chromatography and Spectrophotometry: Fast Green FCF Certified is also used as a tracer or indicator dye in chromatographic and spectrophotometric techniques. It’s bright and stable hue supports precise detection and analysis, making it applicable to various chemical and biological tests.
- Cytological Counterstaining: Apart from its use in histology, Fast Green FCF is also used as a cytological counterstain to enhance the contrast of the cellular structures for easy microanalysis.
Key Features of Fast Green F.C.F. Certified
- Certified Coloring Agent: Fast Green FCF Certified ensures its safety for use. This certification ensures that Fast Green FCF is safe and can be used extensively for industrial and scientific purposes.
- Effective Colorant for Visual Effects: Fast Green FCF Certified is an effective dye and produces a stable, intense green color. The ability to consistently produce brilliant and radiant results makes it essential in various staining areas.
- Stability and Versatility: This dye’s versatility makes it applicable in many fields, including protein staining, chromatography, and spectrophotometric analysis.
General Description
A member of the triarylmethane class of colorants, Fast Green F.C.F. Certified (CAS No. 2353-45-9) is a synthetic, water-soluble dye. Its applications in histology, cytology, microbiology, and analytical chemistry require the use of strong dyes, and this dye is widely used due to its bright, dark green color. Suitable for precise staining procedures and enhancing visualization, Fast Green F.C.F.’s chemical composition is C37H34N2O10S3Na2 (MW=808.85), which makes it a versatile and reliable dye.
If you are looking for where to buy Fast Green F.C.F. Certified (CAS No. 2353-45-9), Dawn Scientific is the most convenient place to get it. We have a complete assortment of histological stains and dyes that your laboratory may need. We understand that the usage of the dye could either be for research, histology, or even industrial purposes, hence, Fast Green FCF (CAS No. 2353-45-9) is offered with all the product details you would need. So, make sure to visit our website so that you can place an order today!
To get the exact pricing details for Fast Green F.C.F. Certified (CAS No. 2353-45-9), it is best to reach out to Dawn Scientific. We are always ready and willing to provide you with the necessary information to assist you with the current pricing and competitive pricing solutions, depending on how many units you require.
About Brand
“TriStains”, Histological Stains/ Biological Stains that offer a range of stains used in Histology, Cytology, Microbiology, and Hematology laboratories.
TriStains meet the highest quality standards and give excellent color performance of desired components of cells and tissue in life science laboratories.
TriStains series products are carefully tested to ensure accurate, reliable, and reproducible results. Our products are available in different packaging sizes to allow you to get all types of stains & Indicators for your specific purposes from a single source.
Why Dawn Scientific?
In addition to our specialty product lines, Dawn Scientific offers a comprehensive range of laboratory supplies and scientific products, including laboratory chemicals, reagents, analytical standards, solutions, buffers, biological stains, indicators, laboratory equipment, instruments, consumables, and much more.
For complete product information, technical resources, and our full catalog, please browse other categories on our website dawnscientific.com.
If you have any questions or would like assistance selecting the right products for your application, our knowledgeable team is ready to help. Contact us at 1-800-DAWN-SCI (1-800-329-6724) or email sales@dawnscientific.com. We look forward to serving your laboratory and research needs.
Frequently Asked Questions (FAQs)
TriStains Fast Green F.C.F. Certified is widely used as a histological stain, protein stain, and cytological counterstain in histology, microbiology, electrophoresis, and biological research applications.
TriStains Fast Green F.C.F. Certified is commonly available in:
- • 5 g
- • 25 g
pack sizes for laboratory and research requirements.
No, this product is generally supplied as a high-purity powder or granular form for preparation of staining solutions according to specific laboratory protocols.
TriStains Fast Green F.C.F. Certified is manufactured using high-purity certified staining-grade material, with certified quality standards for reliable staining performance and reproducible laboratory results. Competitor specifications commonly indicate dye content of ?85–90% or higher.
It provides:
- • Bright and intense green coloration
- • Excellent tissue contrast
- • Reliable protein staining performance
- • Consistent batch-to-batch reproducibility
for research and laboratory applications.
Yes, it is widely used for protein staining in IEF, SDS-PAGE, and native PAGE electrophoresis techniques, providing clear protein visualization and analytical performance.
Yes, Fast Green F.C.F. is commonly used in histology, plant tissue staining, collagen staining procedures, and trichrome staining methods for enhanced tissue visualization.
The product is highly soluble in water and also soluble in glycerin, glycol, alcohol, and ethanol, allowing convenient preparation of laboratory staining solutions.
Yes, TriStains products are carefully validated to ensure accurate color development, staining consistency, reliability, and reproducible laboratory results.
TriStains Fast Green F.C.F. Certified offers certified staining quality, high-purity formulation, strong staining intensity, multiple packaging options, and dependable laboratory performance, making it suitable for histology, cytology, protein analysis, and research applications.








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