Buy Fluorescein Free Acid (2321-07-5) MF: C20H12O5 | MW: 332.31. Get High Quality Fluorescein Free Acid (-) from TriStains at Dawnscientific.com
Fluorescein free acid is a chemical compound that belongs to the family of xanthene dyes. It is a derivative of fluorescein, which is commonly used as a fluorescent tracer and pH indicator.
“TriStains”, Biological Stains that offer range of stains used in Histology, Cytology, Microbiology and Hematology laboratories.
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Applications:
Fluorescent Tracer: Fluorescein free acid is widely used as a tracer or marker in various scientific and medical applications. It can be conjugated to other molecules, such as proteins or antibodies, to track their movement or localization within biological systems. This property makes it valuable in fields like molecular biology, immunology, and cell biology.pH Indicator: Fluorescein free acid is a pH-sensitive dye. It exhibits different colors and fluorescence intensities depending on the pH of the environment. It is often used as a pH indicator in analytical chemistry, biochemistry, and clinical diagnostics. The change in fluorescence can be used to monitor pH changes in solutions, enzymatic reactions, or cellular compartments.Fluorescence Microscopy: Fluorescein free acid is employed in fluorescence microscopy to visualize and study cellular structures and processes. It can be used to stain and label specific cellular components, such as nuclei, cytoskeleton, or organelles. By exciting the fluorescein with specific wavelengths of light, researchers can visualize and study these structures in living or fixed cells.Flow Cytometry: Flow cytometry is a technique used for cell analysis and sorting. Fluorescein free acid, when conjugated to antibodies or other specific molecules, enables the identification and quantification of different cell populations. By labeling cells with fluorescein-conjugated antibodies, researchers can analyze and sort cells based on their fluorescence properties, providing valuable information about cell types and characteristics.Ophthalmic Diagnostic Agent: Fluorescein free acid is used in ophthalmology as a diagnostic agent for various eye examinations. It is commonly used in fluorescein angiography, a technique that involves injecting fluorescein dye into the bloodstream to visualize blood vessels in the retina and choroid. This helps in diagnosing conditions like macular degeneration, diabetic retinopathy, and vascular occlusions.Water Tracing: Fluorescein free acid can be used as a fluorescent tracer in environmental studies and hydrology. It is added to water systems to track the movement and dispersion of water, helping to understand the flow patterns and identify potential pollution sources.
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