Neutral Red is a pH indicator dye that is commonly used in biological and chemical applications. It is a redox indicator that changes color depending on the pH of the solution it is in. When the pH is acidic, the dye appears yellow, while in neutral to slightly basic conditions, it appears red. Neutral Red is primarily used in the laboratory and industrial settings for various purposes.
“TriStains”, Biological Stains that offer range of stains used in Histology, Cytology, Microbiology and Hematology laboratories.
TriStains meet the highest quality standards and give excellent color performance of desired components of cells and tissue in life science laboratories.
TriStains series products are carefully tested to ensure accurate, reliable, and reproducible results. Our products are available in different packaging sizes to allow you to get all types of stains & Indicators for your specific purposes from a single source.
In-addition Dawn Scientific also deals in numerous Laboratory Supplies, Chemicals, Equipment, Instruments, Reagents, Standard Solutions, Buffers, Biological Stains & Indicators and many more, for more information please visit our website www.dawnscientific.com you may also call on 1.-800-DAWN-SCI or email to sales@dawnscientific.com any Query/information we will be happy to help you.
Applications:
Cell Viability Assay: Neutral Red is widely used in cell culture studies to assess cell viability. It is added to the culture medium, where it is taken up by viable cells and accumulates in their lysosomes. The dye is then released into the medium upon cell lysis. By measuring the absorbance of the released dye, researchers can determine the number of viable cells present in a culture. Microbiological Applications: Neutral Red is utilized in microbiology for various purposes. It can be used as an indicator for bacterial growth in culture media, as many bacteria change the pH of the medium as they grow. Additionally, it is employed in various bacterial identification tests, such as the triple sugar iron agar test, to distinguish different bacterial species based on their ability to ferment specific sugars.Histology and Cytology Staining: Neutral Red is employed in histology and cytology staining procedures. It can be used to stain acidic components of cells, such as nucleoli, lysosomes, and mast cells, providing contrast and aiding in the visualization of cellular structures under a microscope. Tissue Culture Applications: In tissue culture, Neutral Red can be used to assess the viability of explanted tissues or to evaluate the efficacy of tissue preservation methods. By staining the tissues with the dye and observing the uptake and release of the dye, researchers can determine the viability and metabolic activity of the tissue samples.Toxicity Testing: Neutral Red is frequently used in toxicity assays to evaluate the cytotoxic effects of chemical compounds or substances. By treating cells or tissues with the test compounds and subsequently assessing the uptake and release of the dye, researchers can determine the potential toxicity or adverse effects of the tested substances.
Neutral Red, 90%
Get High Quality Neutral Red, 90% (553-24-2) from ChemieR at Dawn Scientific. M.F: C15H17ClN4 | M.W.: 288.78, Shop now for reliable and Reproducible results
Biological Stains
High quality chemicals for all of your
laboratory requirements at lower price.
ADDITIONAL INFORMATION
Alternate Names | 3-Amino-7-dimethylamino-2-methylphenazine hydrochloride; Basic Red 5; Toluylene red |
Application | Neutral Red is a common histology stain used to determine cell viability. |
CAS Number | 553-24-2 |
Molecular Weight | 288.78 |
Molecular Formula | C15H17ClN4 |
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
Neutral Red is most commonly used for histological stain for lysosymes but has also been incorporated in investigation of viruses, as a pH indicator, determination of DNA with optical and electrochemical methods, evalutaion of synthetic and naturally derived biomaterials. Neutral red can be electrochemically polymerized and used as a redox mediator. The compound once taken up by cells can be used to acertain cell viability with a excitation maximum at 470 nm and emission maximum at 580nm.
TECHNICAL INFORMATION
Appearance | Powder |
Physical State | Solid |
Solubility |
Soluble in water (50 mg/ml at 25° C), abs. alcohol (1.8%), and cellosolve (3.75%).
|
Storage | Store at 4° C |
Melting Point | 290° C (lit.)(dec.) |
Boiling Point | 497.3° C at 760 mmHg |
Refractive Index | n20D 1.75 (Predicted) |
IC50 : | Internal ribosome entry site (IRES): IC50= 20 µM (human); Huh-7: IC50= 69.26 µM (human); -Arrestin-1: IC50= 8.88 µM (human); Cytoplasmic zinc-finger protein: IC50= 13.00 µM (Caenorhabditis elegans); Apaf-1: IC50= 34 µM (human) |
SAFETY INFORMATION
WGK Germany | 3 |
RTECS | SG1400000 |
PubChem CID | 11105 |
Merck Index | 14:6488 |
MDL Number | MFCD00012651 |
EC Number | 209-035-8 |
Beilstein Registry | 3918740 |
SMILES | CC1=CC2=NC3=C(C=C(C=C3)N(C)C)N=C2C=C1N.Cl |
Reviews
There are no reviews yet.