Buy Sudan Black B Certified (4197-25-5) MF: C29H24N6 | MW: 456.54. Get High Quality Sudan Black B Certified (4197-25-5) from TriStains at Dawnscientific.com
Sudan Black B is a synthetic dye that has been used historically in the laboratory setting for various applications.
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Applications:
Staining agent: Sudan Black B is commonly used as a staining agent in histology and cytology laboratories. It is primarily used to stain lipids, particularly neutral lipids such as triglycerides and lipoproteins. By binding to lipids, Sudan Black B helps visualize lipid-rich structures and lipid droplets within cells.Lipid detection: Sudan Black B staining is often used to detect lipid-containing structures in biological samples. It is commonly employed in research studies involving adipocytes (fat cells), where it can aid in the identification and quantification of lipid content in tissues or cells.Myelin staining: Sudan Black B has also been used to stain myelin in nerve tissue. Myelin is a fatty substance that wraps around nerve fibers and plays a crucial role in facilitating nerve signal transmission. By staining myelin, Sudan Black B can help visualize and study the distribution and integrity of myelin in the nervous system.Compatibility and limitations: Sudan Black B is typically used on frozen or paraffin-embedded tissue sections. It is not compatible with formalin-fixed, paraffin-embedded tissues or with other staining methods that involve deparaffinization. It is important to note that Sudan Black B staining may also interfere with the detection of other cellular components, so it is typically used in combination with other stains for a comprehensive analysis of tissues or cells.Toxicity considerations: While Sudan Black B has been widely used in laboratory research, it is important to handle it with caution. Sudan Black B is considered a potential carcinogen and can be harmful if ingested or inhaled. Proper safety precautions, such as the use of gloves, protective eyewear, and a fume hood, should be followed when working with Sudan Black B.
Sudan Black B Certified
Get High Quality Sudan Black B Certified (4197-25-5) from ChemieR at Dawn Scientific. M.F: C29H24N6 | M.W.: 456.54, Shop now for reliable and Reproducible results
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ADDITIONAL INFORMATION
Alternate Names | Sudan Black B is also known as Solvent Black 3, Fat Black HB, and CI 26150 |
Application | Sudan Black B is a fat-soluble diazo dye which has been used to stain phospholipids and intracellular lipids |
CAS Number | 4197-25-5 |
Purity | 99% |
Molecular Weight | 456.54 |
Molecular Formula | C29H24N6 |
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
Sudan Black B is primarily used for staining of neutral triglycerides and lipids. Also a histochemical stain for chromosomes, Golgi apparatus, and leucocyte granules. Sudan Black B has the appearance of a dark brown to black powder with maximum absorption at 596-605 nm and it stains blue-black. Sudan dyes are a group of lipid soluble solvent dyes often called lysochromes, in structural classification they are diazo dyes. Sudan Black B is not as lipid-specific as some of the other Sudan dyes and may be useful to stain other materials. Sudan Black B is useful for detecting fats that are contaminated with oil and grease. Sudan black B is used in the study of haematological disorders as it will stain myeloblasts, but not lymphoblasts. Sudan Black B can also stain materials ionically; in this case some background staining is to be expected, and interpretation of sections stained with it must take this into account.
TECHNICAL INFORMATION
Appearance | Powder |
Physical State | Solid |
Solubility | Soluble in oils, fats, warm petrolatum, paraffin, phenol, ethanol (10 mg/mL), acetone, benzene, toluene, and hydrocarbon. Insoluble in water. |
Storage | Store at 4° C |
Melting Point | 120-124° C (lit.) |
pK Values : | pKb: 10.88 |
SAFETY INFORMATION
WGK Germany | 3 |
RTECS | SD4431500 |
PubChem CID | 61336 |
Merck Index | 14:8885 |
MDL Number | MFCD00006919 |
EC Number | 224-087-1 |
Beilstein Registry | 723248 |
SMILES | CC1(NC2=C3C(=C(C=C2)N=NC4=CC=C(C5=CC=CC=C54)N= NC6=CC=CC=C6)C=CC=C3N1)C |
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