Buy Amido Black 10B (1064-48-8) MF: C22H14N6O9S2•2Na | MW: 616.49. Get High Quality Amido Black 10B (1064-48-8) from TriStains at Dawnscientific.com
Amido Black 10B is a commonly used synthetic dye that has a deep blue to black color. It is also known by other names such as Acid Black 1, C.I. 20470, and Acid Black 10B. The “10B” in its name refers to its identification number in the Color Index (CI) system.Amido Black 10B is primarily used as a histological and protein stain in various scientific and forensic applications.
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Applications:
Staining Properties: Amido Black 10B has an affinity for proteins, particularly albumin and globulin. It binds to the proteins, forming a stable complex that results in a visible stain.Protein Staining: In protein gel electrophoresis techniques like SDS-PAGE, Amido Black 10B is used to visualize protein bands. After the electrophoresis process, the gel is stained with Amido Black 10B, which binds to the proteins present in the gel, allowing researchers to visualize and analyze the protein bands.Sensitivity: Amido Black 10B is known for its high sensitivity, allowing the detection of even small amounts of protein. This property makes it useful in applications where low protein concentrations need to be detected.Bloodstain Detection: In forensic science, Amido Black 10B is employed to enhance the visibility of bloodstains. It reacts with the heme component of blood, resulting in a dark blue or black color that makes it easier to locate and document bloodstains at crime scenes.Limitations: While Amido Black 10B is a widely used staining agent, it has some limitations. It may not stain all types of proteins equally, and the intensity of staining can vary depending on the protein composition. Additionally, it may interfere with downstream applications such as mass spectrometry analysis.
Amido Black 10B
Discover Amido Black 10B (CAS: 1064-48-8), also known as Acid Black 1, at Dawn Scientific. Molecular formula: C22H14N6O9S2•2Na. Molecular weight: 616.49.
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ADDITIONAL INFORMATION
Alternate Names | Acid Black 1; Amido Black 10B; Buffalo Black NBR, Naphthol Blue Black |
Application | Naphthol Blue Black is a histological, protein, and nucleic acid dye used in electrophoresis |
CAS Number | 1064-48-8 |
Molecular Weight | 616.49 |
Molecular Formula: | C22H14N6O9S2•2Na |
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
Naphthol Blue Black is an important and commonly used industrial dye. This dye is also used in rapid staining of protein bands on nitrocellulose membranes such as Western blots. Naphthol Blue Black can be utilized as a nucleic acid stain in SDS-Page gels. Additionally this compound has been untilized in cell proliferation assays and histochemical studies.
TECHNICAL INFORMATION
Appearance | Powder |
Physical State | Solid |
Solubility | Soluble in water (10 mg/ml at 25° C), and ethanol. |
Storage | Store at room temperature |
Refractive Index | n20D 1.77 (Predicted) |
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