Liquid/solid chromatography is a technique used to separate a sample into its individual parts. This separation occurs based on the chemical or physical interactions of the sample with the mobile and stationary phases. Because there are many stationary/mobile phase combinations that can be employed when separating a mixture, there are several different types of chromatography that are classified based on the physical states of those phases. Liquid-solid column chromatography, the most popular chromatography technique, features a liquid mobile phase which slowly filters down through the solid stationary phase, bringing the separated components with it.

Liquid column chromatography, the most popular chromatography technique & the one discussed here, features a liquid mobile phase which slowly filters down through the solid stationary phase, bringing the separated components with it.

This method is similar to partition chromatography only in that the stationary phase has been replaced with a bonded rigid silica or silica-based component onto the inside of the column. Sometimes the stationary phase may be alumina. The analytics that are in the mobile phase that have an affinity for the stationary phase will be absorbed onto it and those that do not will pass through having shorter retention times. Both normal and reverse phases of this method are applicable.

This is a type of chromatography that is applied to separate and determine ions on columns that have a low ion exchange capacity. This is based on the equilibrium of ion exchange between the ions in solution and the counter ions to pair with the oppositely charged ions that are fixed to the stationary phase. This stationary phase would either have positive of negative functional groups affixed to it, usually sulfonate (-SO3-) or a quaternary amine (-N(CH3)3+), being a cation and anion exchanger respectively.

A buffer is a solution of a weak acid and its conjugate base, or a weak base and its conjugate acid. Buffers reduce the influence of hydrogen ions, hydronium ions, and hydroxide ions, resulting in less pH fluctuations including dilution. In reversed-phase mode, the pH tolerance range of classic silica-based packing materials is 2-8. The choice of buffer is usually determined by the desired pH. The pKa of the buffer must be close to the desired pH, as pH is easiest to control at the pKa. The rule of thumb is to choose a buffer with a pKa value <2 units of the desired mobile phase pH.

In this method, both the stationary phase and the mobile phase are liquid. The stationary phase liquid would be an immiscible liquid with the mobile phase.

Dawn scientific products are certified by ISO 9001:2015. Our various brands such as cUSP (Ready to use Test Solutions), ChemieR (For High purity solvents, Inorganic Reagents, Acid & base), Bluster (For Gas Chromatography), EKS (For Electronic), KAPPAA (Peptide Synthesis) & many more. Fulfilled all your needs for Institutes, Universities & school for highly professional labs.

We assure you our product range delivered you accurate, consistent & reproducible results in all testing. In-addition Dawn scientific had ensured to provides you a quality chemical product, biological stains & indicators, cUSP Solutions, High Purity solvents, GC & GC-HS Grade & complete solution for laboratory such as (Microscopes, Glassware, Plasticware, Stopwatches, Timers, Filtration, Sanitizing products, Weight machines, Thermometers etc.)

• Ready to use solution
• 100%  Recommended by Professional.
• Product COA/SDS available
• Labelled with minimum shelf life/batch number
• Follow standard disposal

Although Dawn Scientific provide you with the best option for single & Bulk quantity, custom size packing as per your requirements in our store. We 100% accept extensive orders & give priority arrangement to delivery on time.

[wpforo]